Giuseppe Avella

Background: BSc. Biomedical Sciences (2011) – University of Naples Federico II (Scholarship Holder from 2008 to 2011); MSc. Molecular Biology (2014) University of Urbino (Scholarship Holder from 2011 to 2013) 

Research Project: Investigating the role of fatty acids in CHO cell culture and the development of novel genome engineering tools.

 Enhancing Chinese Hamster Ovary (CHO) cells productivity of recombinant proteins has been an area of intense research over the last two decades to meet the rising global market demand. The most common approach has been to adapt the media formulation and the bioprocess design to boost large scale production. The project focuses on engineering CHO cells lipids metabolism pathways while growing in lipids rich media formulations evaluating possible recombinant biotherapeutic protein production enhancements. The modulation of gene expression allows to regulate the activity of a protein directly before its transcription. Most of the genome engineering tools are based on the Watson-Crick base pairing. In this project, we focus as well on possible alternatives based on the combination of Hoogsteen base pairing and different DNA modifications to avoid their degradation by nucleases in the cells, while preserving the binding stability.

Funding:  H2020-MSCA-ITN-2014 (ETN) ClickGene. Grant agreement No 642023

Research Gate profile: https://www.researchgate.net/profile/Giuseppe-Avella

Linkedin Profile: https://www.linkedin.com/in/giuseppe-avella-28597549/